Improving genome coverage in SARS-CoV-2 sequencing
Time & Location
About the Event
Currently the most widely used protocol for SARS-CoV-2 library preparation is based on the ARTIC Network’s nanopore sequencing protocols for real-time detection of viral outbreaks, such as Ebola and influenza. As SARS-CoV-2 infections spread, the ARTIC Network nCoV-2019 (i.e., SARS-CoV-2) sequencing protocol was quickly adapted for Oxford Nanopore Technologies® (ONT) sequencing. By applying balanced primer pools, optimized reagents and master mixes within a streamlined workflow for both ONT and Illumina® sequencing platforms, we have improved uniformity of genome coverage. These improvements are available as a complete workflow in the NEBNext® ARTIC SARS-CoV-2 Library Prep Kits.
During this webinar, we will discuss current efforts by researchers and public health experts to monitor SARS-CoV-2 through whole-genome sequencing. We will describe NEB’s continuing efforts to optimize targeted amplicon sequencing and genome coverage across established and emerging SARS-CoV-2 variants to provide researchers around the globe with a sensitive, reliable, and rapid sequencing approach to continue monitoring and addressing this viral pandemic.