Wed, Dec 09|
Fast and Efficient Extraction of High Molecular Weight DNA – Overcoming a Bottleneck for Long Read Technologies
The need for very high molecular weight (HMW) DNA is growing quickly as long read sequencing and other emerging long read technologies become increasingly popular.
Time & Location
Dec 09, 2020, 11:00 AM PST
About the Event
The need for very high molecular weight (HMW) DNA is growing quickly as long read sequencing and other emerging long read technologies become increasingly popular. However, the extraction of HMW DNA has been a bottleneck for these applications, given the complex and lengthy workflows that often take several days to produce usable DNA. Faster methods have emerged, but lengthy protocols, low purity, difficulty dissolving the DNA and high prices remain significant challenges. Here, we will present a novel approach to HMW DNA extraction that utilizes large glass beads and optimized buffer chemistry, resulting in a simple workflow that enables researchers to quickly purify high quality HMW DNA. The Monarch HMW DNA Extraction Kits can be used to extract highly-pure HMW DNA from cells, blood, tissue and bacteria in high yields for use in long read applications. The size of purified DNA ranges from 50 kb up to several megabases; size can be tuned by varying the speed at which samples are agitated during lysis, a unique feature to this workflow. The use of glass beads enables easy handling, extremely efficient elution, high recovery, and easy dissolving of DNA, which is ready to use approximately one hour after extraction. In this webinar, the product developer, Giron Koetsier, Ph.D., will introduce the technology, walk through the workflow, and share some long read sequencing data produced with purified HMW DNA.